The procedures are compliant Seattle-King County regulations apply to autoclaves that treat biohazardous waste. Add chlorine bleach to equal a final concentration of 10 percent bleach. EH&S provides consultation and training for on-site or off-site treatment of sharps and biohazardous waste prior to disposal. approved bleach. II. Liquid disinfectants can be generally classified as halogens, acids, alkalis, heavy metal salts, quaternary ammonium compounds, phenolic compounds, aldehydes, ketones, alcohols, and amines. from spinning down cell cultures. Create a hazardous waste tag and request a pickup (, Attach a waste tag and collect waste separately so that bleach or acidic solutions are not accidentally added to sample preparation kit waste, Visit Water Planning and Stewardship Website for information on Stanfords. Basic information is also provided in Laboratory either routine weekly pickup, or a one-time pickup. Rinse with clean water. Norval, G.A. An example is standard household bleach diluted to the final concentration of 5250ppm (10%). If antibiotics are used that are not on these lists, contact EHS for disposal Prepare a fresh working dilution of sodium hypochlorite weekly and indicate the preparation date on the bottle. Employee Illness and Injury Reporting Tool, Supervisor Illness and Injury Reporting Tool, 50 Medical Center Way, San Francisco CA 94143, 2023 The Regents of the University of California. Liquid blood products can be treated with bleach to reduce the . For more information, contact EPS at eps.hazdisposal@utoronto.ca or 416-978-7000. https://ehs.utoronto.ca/our-services/environmental-protection-services/, Online Accident/Incident eForm for Employees, Online Accident/Incident eForm for Students, Contractors and Visitors, Training Matrix: Admin & Facilities Staff, Basic Health and Safety Awareness Training, Respiratory Protection Training and Fit-Testing, Environmental Health & Safety Program Policies, Procedures and Guidelines, Laboratory Hazardous Waste Management and Disposal Manual, https://ehs.utoronto.ca/laboratory-hazardous-waste-management-and-disposal-manual/chemical-waste-disposal/, 215 Huron Street - 7th Floor, Toronto, Ontario, Canada M5S 1A2. Wear rubber or non-porous boots, gloves, and goggles to protect yourself while cleaning. 1. Add the bleach solution in equal volume to your liquid biowaste. Place the autoclaved items in the designated biohazard waste area. Please visit theBiohazardous and Medical Waste Overview for campus policies concerning biohazardous waste disposal for solids. Autoclave cost centers include: If you do not have access to an on-site autoclave or an autoclave cost center, biohazardous waste can be collected by a UW waste contractor for off-site treatment and disposal. BSL-1P material can be composted or desiccated, according to IBC approved protocols. Recommended working dilution: 5250 ppm (1:10 dilution of household bleach of 5.25% sodium hypochlorite), Recommended for floors, spills (inactivating liquid specimens), bench tops and contaminated clothing. 4.10 Biological Waste Management - Virginia Tech Be securely closed before removal from point-of-use, Have NO chemical or liquid waste placed within (with the exception of Vacuum-assisted aspiration devices (See Section 3.13.) Always review the Safety Data Sheet (SDS) and the manufacturers guidelines to determine the chemical compatibility of chemicals or proprietary material with the deactivating agent. In addition it includes Risk Group 1 contaminated material from Recombinant DNA procedures. Bleach is incompatible with many other chemicals. Line each RMW box with 1-2 red RMW plastic liner bags. procedures required by the West Virginia Infectious Medical Waste Rule, 64 Media wastes containing the following HEAT SENSITIVE antibiotics can be autoclaved, contacted, infectious material. For extracted teeth, arrange for off-site incineration. Forms toxic chlorine and chloramine gases. this purpose, and spray it with appropriate disinfectant prior to transport on a Dispose of as liquid chemical waste. Biosafety: How to Disinfect Tissue Culture Media in Vacuum Flasks - Blink Bleach irritates mucous membranes, the skin and the respiratory system. After the required exposure time in the BSC, the liquid waste plus bleach may black bags, or in RMW. pasteur pipettes, or any other object with a sharp edge. Sodium hypochlorite, the active ingredient in chlorine bleach, is routinely used in the laboratory to decontaminate surfaces and equipment or deactivate biological materials by inactivating vegetative bacteria, fungi, lipid and non-lipid viruses, and other liquid specimens. Stanford University, Stanford, California 94305. Learn about different methods of decontamination used in research environments. tightly or opened completely. antibiotics, and once decontaminated, this waste must be discarded as Regulated Try not to breathe in product fumes. Contact them directly for more information. Biohazardous Waste | Environment, Health and Safety Interlock UV lamps used for space decontamination with the general room or cabinet illumination, so turning on the lights turns off the UV. Do not use bleach on electronic equipment, optical equipment or unpainted stainless steel, Undiluted bleach and other disinfectants must not go down the drain or be mixed with other materials, Only 1:10 dilutions of bleach that have been mixed with adequate levels of protein (such as those found in tissue culture media containing fetal bovine serum) can be poured down the drain, Undiluted bleach is substantially more reactive than diluted bleach, and has even been reported to generate toxic gases such as cyanogen and chloramine when mixed with Luria broth in a ~1:1 ratio, Use stock or working bleach solutions in a well ventilated area, Work in a certified chemical fume hood when using volumes greater than 1000mL, Purchase and use the lowest volume and concentration necessary, Do not use bleach in diluted concentrations greater than 10% unless working with prions, Avoid contact with eyes, skin, and clothing, Verify the SDS and manufacturers guidelines for chemical compatibility before mixing bleach, Never mix bleach with incompatible chemicals, an unknown chemical, or mixture. If the extracted teeth contain amalgam, contact EH&S Chemical Waste for pick-up and disposal. Liquid biohazard waste may be treated either by autoclaving or chemical inactivation. Biohazardous Waste: also called infectious waste, is waste contaminated with potentially infectious materials or biohazards. Proper concentrations Precautions: Monitor wet and dry heat sterilizers on a regular basis using appropriate biological indicators [spore strips]. EH&S Shipping Regulated Medical Waste Training is required for all personnel who will package and ship waste. cart to the autoclave room. Common examples of sharps encountered in the research setting include: Solid biohazard waste includes any solid object that came in contact, or potentially If you plan to generate mixed waste (i.e., biohazardous and radioactive), please contact EH&S first. Hypochlorite concentration in household bleach varies by manufacturer. The Centers for Disease Control and Prevention (CDC) cannot attest to the accuracy of a non-federal website. The containers must be labeled with the international biohazard symbol on all sides and labeled with the words, Incineration Only. PDF STANDARD OPERATING PROCEDURE Safe and Correct Way to Use Bleach (Sodium trash. Chlorine compounds have some effect in inactivating bacterial spores: Refer to the Biosafety Manual for additional information and guidance on selecting appropriate disinfectants, or for appropriate bleach use with prions and prion-like proteins. placed within a bag or sealed container in the BSC, which is sprayed with Do not attempt to re-open or re-use a previously locked container. Bleach solutions for disinfection should be prepared daily. How to Use Bleach to Clean - The Spruce Biohazard and Medical Waste - University of California, Santa Cruz If autoclaving, use verification devices. Remove can labels. Media wastes containing the following HEAT-STABLE antibiotics cannot be autoclaved broths, human blood, and human body fluids. If transporting, close and seal containers, and place in a leak-proof secondary container. with West Virginias Infectious Medical Waste Rule (64 CSR 56). Biological Waste Guide | Environmental Health and Safety Install the bulb with gloved hands to prevent oil build up. Plan for disposal before generating biohazardous waste. Liquid disinfectant effectiveness varies with the organism, concentration, contact time, and other conditions of use. Use of bleach solutions with lower hypochlorite concentrations will not provide the proper level of disinfection. Collect the waste in a well-labeled autoclavable container (containing no bleach), then. The active ingredient in bleach, sodium hypochlorite, is not a hazard to the environment and can be disposed of into the wastewater stream. UC San Diego 9500 Gilman Dr. La Jolla, CA 92093 (858) 534-2230, Review the Biohazardous Waste Disposal Guidelines for Liquid Waste, Review the Biohazardous Waste Disposal Guidelines for Solid Waste, Review the Biohazardous Waste Disposal Guidelines for Sharps Waste, Disposal of Unwanted Equipment through Surplus Sales, waste derived from the production of biological agents, used sharps, contaminated animal carcasses, body parts, excrement and bedding. Wear rubber or other non-porous boots, gloves, and eye protection. Orange bags are required per 64 CSR 56, WV Infectious Medical Waste Rule. Bleach is a water-based solution commonly used as a disinfectant. Instead, contact campus EMF (. EHS supplies containers that meet the requirements of being closeable, Follow the same procedure for Outside Disposal of Infectious Medical Waste, with Biosafety SOP: Sharps Handling and Disposal Procedures, Proper Disposal of Sharps at Animal Facilities, Waste: Do not mix bleach with biological materials in trizol. Biological Waste Guide | Environment, Health and Safety 1. Blot up bleach solution with disposable towels. CDC is not responsible for Section 508 compliance (accessibility) on other federal or private website. PDF Biomedical Waste Disposal Guide - The University of Alabama at Birmingham Steam sterilization with an autoclave effectively inactivates most infectious agents. Put on protective gear. Biohazardous Waste Disposal Guidelines. Non-food contact surfaces that do not soak up water and that may have touched floodwater. the autoclave will need to be examined for problems. Close the boxs top and seal X3 on center seam with wide cellotape, then tape Close the container shut when the fill-line on the side of the container is reached. Decontamination is a term used to describe a process or treatment that renders a medical device, instrument, or environmental surface safe to handle. 2011 Virginia Polytechnic Institute and State University | Website Feedback. For additional information on biohazardous waste or biological safety, please refer to UCSD BiosafetyProgram overview. Procedure: Disposing Liquid Biohazardous Waste Using the Sanitary Sewer Drain Treat the waste in disinfectant using one of the following methods: 1:10 dilution of household bleach for at least 30 minutes Commercial disinfectant following manufacturer's instructions for appropriate concentration and contact time. Laboratory personnel and principal investigators (PIs) are responsible for identifying, packaging and properly decontaminating biohazardous waste, including all recombinant or synthetic DNA/RNA waste, before disposal. Make disposal arrangements before obtaining human or nonhuman primate pathological samples. For site-specific information, see Biohazardous Waste Flow Charts. Bagged waste must be well contained, allowing adequate time for breakdown autoclave rooms) for biweekly removal by campus EMF. All rights reserved. the following exception: Liquid biohazard waste is any liquid or fluid, over approximately 15ml, which potentially If all red "collection bins" in the buidling are full, email. These wastes require incineration prior to disposal. All sharps must be placed in a biohazard sharps container. cellotape. The preferred method of solid biohazard waste collection and disposal for both Infectious has contracted Stericycle for solid biohazard waste disposal. BSL-2: Collect in an ORANGE autoclave bag with a Biohazard symbol. Forms chloroform, hydrochloric acid, chloroacetone or dichloroacetone. by heat, and are then environmentally safe to go into the domestic sewer. Liquid biohazardous waste includes Risk Group 2 contaminated cell culture media and solutions, blood and bodily fluids containing blood. Safety Management System. Only mercury-free bleach can be poured down the drain, all other chemicals must be collected in containers for chemical waste disposal. Bleach is not stable at dilute concentration. of bleach to liquid waste. For disposal, transfer the red bags to a red biohazard tub labeled Incineration Only. If the weather is hot and humid, take plenty of breaks in a cooler place and drink lots of water. Allow to air dry. Sharps Disposal for non-sharps and sharps biohazardous waste Basic procedure: Collect biowastes at the laboratory level in designated biohazard-labeled cans per current biowaste collection procedures. same method. Use water to dilute the bleach as you pour it down the drain. BSL-2 It is acceptable to autoclave BSL-2 waste containing heat-stable or heat-labile Decontamination by bleach is a less reliable decontamination method because of the opportunities for a failed result if the bleach used is 1) expired, 2) present in the wrong proportion to liquid waste volume, and 3) not given sufficient contact time before disposal. Media with additives such as growth factors, metals or other chemicals must At end of a work session or when bag is 2/3 full, securely close the bag, place UC San Diego 9500 Gilman Dr. La Jolla, CA 92093 (858) 534-2230 Some examples of approved sharps containers include: Sharps containers must be kept in the immediate vicinity of the sharps usage. If you're choosing to pour the bleach down your kitchen drain, turn on the faucet first. Download the. Bags must be autoclaved daily when possible, or as soon as an autoclave is available. Allow the mixture to sit for at least 10 minutes to allow adequate disinfection. Lab workers who produce larger volumes of tissue/carcass waste or other types NOTE: Permission to use greenhouse steam box Regulated Medical waste. For disposal into a sanitary landfill, the autoclaved biohazard bag should be bleach to yield a 1:5 dilution. Wash cans with soap and warm, clean water. Precautions: UV can cause burns to the eyes (photokeratitis) and skin of people exposed for even a short period of time. disinfectant other than bleach is used, it must be disposed of as chemical waste. For more information on cleaning and sanitizing with bleach and how to make water safe for drinking, see CDCs pages on Household Cleaning and Sanitizing and Make Drinking Water Safe. Refer to "Off-site sterilization" above. Label the red, biohazardous sharps container with UCSD identification and generatorinformation prior to any addition of waste: Do not place free liquids, such as full syringes, in sharps containers. present (e.g., tiny pieces of dissected unfixed tissue) can dispose of that Organisms must be directly exposed to the UV light; dirt, dust, and shadows can shield organisms, limiting UV lamp effectiveness. These may be animal, mineral, medical, bacterial, chemical, or infectious, and most of these require specific methods of disposal. . Following autoclaving or chemical inactivation: Chapter 1: Introduction and Administration, Chapter 2: Institutional Biosafety Committee, Chapter 6: Occupational Health and Exposure Response, Chapter 8: Decontamination, Disinfection and Spill Response, Chapter 9: Transporting and Shipping Biohazards, Chapter 10: Personal Protective Equipment for Biohazards, Chapter 11: Biosafety for CSW and FM Employees, Building Safety - Facility Code Compliance, Control of Hazardous Energy (Lockout/Tagout), Powered Industrial Truck (Forklift) program, Hazard Comm/Hazardous Waste/Lab Safety Training, Online Hazardous Waste Training Attendance Records, West Virginia Infectious Medical Waste Rule (64 CSR 56), Infectious Medical Waste Rule (64 CSR 56), Institutional Accounting, Reporting, & Analysis, Sponsored Projects & Property Administration, Planning, Design, Construction and Scheduling, Procurement Contracting and Payment Services, Cultures and stock of microorganisms (Risk group 2 and higher), Human Blood and tissue samples (including cell lines), Recombinant nucleic acids requiring BSL2 or higher containment (including transgenic Liquid Biohazardous Waste | UMN University Health & Safety Becauserecombinant or synthetic DNA/RNA is considered a biohazard, those tips do need to be autoclaved. How to Identify, Label, Package and Dispose of Biohazardous and Medical Copyright 2023 Regents of the University of California. Lab workers who produce a minimal volume of tissue material with no chemical Using Bleach to Decontaminate Liquid Biohazardous Waste, Liquid biohazardous waste may be decontaminated by adding bleach to the liquid waste until a 1-3% concentration of sodium hypochlorite (NaClO) is achieved. Contact EHS to determine the best disposal method. pathogenic to healthy humans. If the second cycle fails to change the color of the tape, *Download form and open in Adobe for full functionality. All infectious materials and all contaminated equipment or apparatus should be decontaminated before being washed, stored, or discarded. Allow the 10% bleached waste solution to sit for 30 minutes prior to drain disposal. Refer to the radioactive waste page for more information. disinfectant before removal from the BSC. Place the full, locked sharps container in your building's designated biohazardous waste collection area (e.g. Once the liquid has been decontaminated it can be poured down the drain. 3) Ensure the contents are mixed thoroughly. Decontaminated BSL-1 waste can be discarded into regular trash, or disposed of in Prepare a fresh 10% percent bleach solution. In order to minimize exposure to biohazards, all bags must be closed and tied off before pickup. Tightly recap bleach bottle and store in a corrosive-resistant container. Tape the lid closed. Disinfection refers to the elimination of virtually all pathogenic organisms on inanimate objects and surfaces thereby reducing the level of microbial contamination to an acceptably safe level . sharps collection and disposal, but must be well labeled. Can also autoclave and sewer. Typical uses: Sterilization of glassware by dry heat can usually be accomplished at 160170C for periods of 2 to 4 hours. Disposed sharps cannot extend above the "FILL" line. It is critical to read and follow the safety instructions on any product you use. bagsthey disintegrate in high heat. Pathological waste includes animal and human tissues, animal carcasses, Risk Group 2+, and Risk Group 3 materials. Typical uses: Autoclaving is a dependable method of sterilizing laboratory equipment and decontaminating biohazard wastes. Apply the bleach solution onto a surface and allow it to sit for 10 minutes. household bleach added 3-5 red biohazard waste bags for biohazard spill debris. Place in suitable liquid handling container. Sodium Hypochlorite (Bleach) - Stanford Environmental Health & Safety Also, if your lab does any biohazardous work, it is easier to treat all pipets and tips as biohazardous so that you do not have to make a decision for each tip; instead, they all go as biohazardous waste. Sharps waste includes needles, syringes, broken glass, glass slides, cover slips, razors, scalpels, knives, Once you're finished, let the water run for another . Waste container boxes must not be overfilled. PDF Biohazard Spill Standard Operating Procedure - University of Guelph One of the simplest measures you can take to protect yourself (and others) inside and outside of the laboratory is washing your hands. Note that Clorox bleach is the only EPA Mix 1 cup (240 mL) of bleach in 1 gallon of water. Items can be removed from the BSC after they have been decontaminated with an Each individual working with biohazardous material should be responsible for its proper handling. Collect in clear autoclave bags or other bags/containers appropriate for the Waste should stored in grey biohazard collection bins with tightly sealed lids. Check buffer and kit specs before disinfecting with bleach. The final concentration of liquid biohazardous waste to bleach should be 10:1 or 10% bleach. All sharps containers are to be autoclaved using the same cycle as used for biological Senior Vice President for Finance and Operations, Thompson Center for Environmental Management treatments that lyse cell membranes renders bioagents non-viable and no longer Medical Waste---the same disposal method as BSL-2 plates containing no antibiotic. Bags must be loosely closed with a knot or twist tie. Unfixed tissue that will not be returned to the body is considered biohazard waste and is to be kept to a minimum and subsequently disposed of in a manner that will allow . Place contaminated towels in plastic bags marked with biohazard sign. Typical uses: Ultraviolet radiation is typically used to reduce levels of airborne microorganisms and maintain good air hygiene in air locks, animal holding areas, ventilated cabinets, and laboratory rooms. PDF Biohazardous Waste Disposal Guide - Dartmouth It is suitable for the destruction of viable organisms on impermeable non-organic surfaces such as glass, but it is not reliable in the presence of shallow layers of organic or inorganic materials which may act as insulation. Liquid biological waste includes cell cultures, contaminated medias or supernatents Forceps or tongs for picking up broken glass or other sharps. Incineration or cremation is required for human pathological waste and nonhuman primate carcasses and body parts. Ionizing radiation will destroy microorganismsbut is not a practical tool for laboratory use. Yes. Add household bleach to the liquid to be decontaminated until a 10% (1:10) concentration of the household bleach is achieved. solid waste and then disposed of in the same manner as BSL-2 waste, i.e., as agents such as bleach and reactive sterilants such as glutaraldehyde. Some brands of bleach, however, contain trace amounts of mercury from the manufacturing process and should not be used. Mixing bleach with incompatible chemicals can produce toxic gases that can potentially damage the eyes, skin, lungs, vocal cords, nervous system, liver, and kidneys. Disposal of biohazardous waste within the grey collection bins (and subsequent pick-up by campus EMF) is the standard process for all campus laboratories. Don't mix bleach with ammonia or acids. The UW Medicine Willed Body Program can provide guidance on items that may require interment or cremation. 1. BSL-1: Collect in a non-colored autoclave bag with no Biohazard symbol. At the end Examples include: Discard all sharps used with biologicals into lidded, rigid, labeled Bio-sharps Can also autoclave and sewer. Potentially infectious cell or tissue culture waste media constitute a biological waste that must be disinfected prior to disposal. Do not store bleach on the floor. WVU The UV-C band of ultraviolet (UV) radiation contains wavelengths (250-270 nm, 265 is optimum) that effectively destroy most microorganisms in air and water and on surfaces. Hazards Bleach irritates mucous membranes, the skin and the respiratory system. . Autoclave cost centers charge a fee for autoclaving and disposal of waste. Autoclaving is the preferred method. deactivate biologicals, then dispose of as liquid chemical waste. Request a Hazardous Waste Pickup: or EHS Assistant. Copyright Complaints, Spill Response Standard Operating Procedure (SOP), Brethericks handbook of reactive chemical hazards, https://www.ncbi.nlm.nih.gov/books/NBK537213/, https://www.atsdr.cdc.gov/phs/phs.asp?id=51&tid=16. Cold water should be used for dilution as hot water decomposes the active ingredient, sodium hypochlorite, rendering it ineffective. Allow the mixture to sit for at least 10 minutes to allow adequate disinfection. Refer to the chemical waste page for more information. The risk can be directly through infection or indirectly through damage to the environment. Never re-cap needles or scalpel blades before disposal into a sharps container. Please follow the guidelines for each . Option #2: Chemical Inactivation. For example, you can add 1 part bleach (containing 10% NaClO) into 9 parts liquid biohazardous waste, or 300 ml of household bleach into 700 ml of biohazardous waste in a 1 L container. Use proper shielding when UV lamps are in use. Below are the most important safety guidelines when using sanitizing products: Use regular, unscented household bleach with a sodium hypochlorite concentration between 5% and 9% (this is the most common concentration in the United States) and follow the instructions in the chart below. Add 10% bleach solution over the spill spot and let stand approximately 30 minutes to ensure adequate germicidal action. The typical shelf-life of bleach with a 4-6% concentration is 3-5 months. Final concentration should be 10% bleach. Cleaning and sanitizing your household after an emergency is important to help prevent the spread of illness and disease. Decontaminated BSL-1 waste can be disposed of in regular trash cans lined with You also can wear a face mask when cleaning with bleach to reduce the amount of fumes you breathe in. 3.17.4. examples below), preferably with a foot operated, self-closing lid. more expeditiously. WEEKENDS OR BREAKS. Collect in a container that cannot be punctured such as a pipette box/keeper or a sturdy cardboard box lined with a biohazard bag. . The following information describes the established lab waste streams and management All rights reserved. equipped with a closeable lid. This waste may be autoclaved or disinfected with bleach and flushed down the drain. Medical Waste and Low-Risk Biohazards is through an outside disposal company. appropriate disinfectant, such as being sprayed with 70% ethanol, or they can be All liquid biohazardous waste must be sterilized using bleach or other approved liquid disinfectant. What is Biomedical Waste? Laboratory waste, including, but not limited to, all of the following: Human or animal specimen cultures from medical and pathology laboratories. Medical Waste and Low-Risk Biohazardous Materials. All solid medical waste is picked up by EH&S and treated offsite for disposal. Liquid biohazardous waste must be treated before disposal via sanitary sewer and cannot be disposed of as solid waste. Liquid biohazardous waste containing hazardous chemicals should be disposed of as a chemical waste and never treated with bleach. Biohazards: Any biological agent that has the potential to cause harm to people, animals, plants, or the environment. Place the full, secured red bags in your building's designated biohazardous waste collection area (e.g.
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